Table 4.

Gene expression in five organs of rainbow trout fry at 4 h, day 2 and day 7 post-initial vaccination

	4 h	Day 2	Day 7
Gill
TLR-2	1.835 ± 0.48	2.427 ± 0.73	1.776 ± 0.73
C3	0.878 ± 0.61	1.06 ± 0.76	1.215 ± 0.66
IL-1β	0.654 ± 0.41	0.512 ↓ ± 0.34	0.651 ± 0.43
IgT	1.041 ± 0.64	1.096 ± 0.72	0.713 ± 0.50
IgM	0.713 ± 0.34	1.344 ± 0.85	1.088 ± 0.66
CD4	0.914 ± 0.66	0.975 ± 0.83	0.922 ± 0.70
CD8	0.81 ± 0.58	0.76 ± 0.38	1.071 ± 0.58
Hind-gut
TLR-2	1.808 ± 0.55	1.5 ± 0.86	0.562 ± 0.30
C3	0.444 ± 0.09	0.485 ± 0.163	0.611 ± 0.15
IL-1β	1 ± 0.51	1.018 ± 0.54	0.678 ± 0.37
IgT	0.742 ± 0.32	2.050 ↑ ± 1.23	0.23 ± 0.02
IgM	1.381 ± 0.72	2.035 ↑ ± 1.16	0.51 ± 0.15
CD4	1.126 ± 0.93	1.476 ± 1.01	0.853 ± 0.46
CD8	0.873 ± 0.54	0.748 ± 0.37	0.538 ± 0.25
Skin
TLR-2	2.08 ± 0.89	4.348 ↑ ± 1.82	1.611 ± 0.53
C3	1.038 ± 0.73	0.958 ± 0.83	1.093 ± 0.85
IL-1β	1.019 ± 0.59	0.475 ± 0.23	0.971 ± 0.54
IgT	1.183 ± 0.75	1.322 ± 0.54	0.491 ± 0.24
IgM	0.73 ± 0.53	1.729 ± 0.77	1.1 ± 0.34
CD4	0.772 ± 0.46	1.272 ± 0.88	0.974 ± 0.71
CD8	0.679 ± 0.34	0.711 ± 0.31	0.713 ± 0.42
Head kidney
TLR-2	1.031 ± 0.45	0.918 ± 0.56	0.791 ± 0.50
C3	0.677 ± 0.10	1.444 ± 0.43	5.26 ± 0.67
IL-1β	1.819 ↑ ± 0.90	1.001 ± 0.42	1.347 ± 0.64
IgT	0.995 ± 0.51	1.199 ± 0.85	2.189 ± 0.20
IgM	0.988 ± 0.71	0.784 ± 0.53	0.654 ± 0.42
CD4	0.944 ± 0.66	1.128 ± 0.83	1.191 ± 0.83
CD8	0.788 ± 0.56	0.426 ↓ ± 0.21	0.947 ± 0.47
Spleen
TLR-2	1.823 ± 0.54	2.017 ± 0.687	1.037 ± 0.37
C3	0.793 ± 0.39	1.205 ± 0.63	0.67 ± 0.32
IL-1β	2.494 ± 0.98	0.734 ± 0.44	1.08 ± 0.75
IgT	0.898 ± 0.41	1.572 ± 0.737	1.23 ± 0.46
IgM	1.184 ± 0.60	0.734 ± 0.34	0.649 ± 0.41
CD4	0.869 ± 0.63	0.935 ± 0.61	0.782 ↓↓ ± 0.61
CD8	0.701 ± 0.46	0.469 ± 0.24	0.554 ↓ ± 0.36

Fold change of genes in vaccinated groups compared to controls ± SE.

↑ and ↓ indicate significant up-regulation and down-regulation relative to control respectively (p < 0.05). ↓↓ indicate significant up-regulation and down-regulation relative to control respectively (p < 0.01).