REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alexa488 conjugated anti-puromycin	Millipore	Cat# MABE343-AF488; RRID: AB_2566826
Rabbit anti-β-actin	Abcam	Cat# ab8227; RRID: AB_2305186
Rabbit anti-β-catenin	Sigma-Aldrich	Cat# C2206; RRID: AB_476831
HRP-conjugated secondary antibodies	Abcam	Cat# ab97080; RRID: AB_10679808
Chemicals, Peptides, and Recombinant Proteins
Cycloheximide	Sigma-Aldrich	Cat# C4859
Anisomycin	Sigma-Aldrich	Cat# A9789
Cy5-UTP	PerkinElmer	Cat# NEL583001EA
Biotin-11-UTP	PerkinElmer	Cat# NEL543001EA
1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate (Dil)	Thermo Fisher Scientific	Cat# D282
Critical Commercial Assays
RNeasy Mini Kit	QIAGEN	Cat# 74104
SuperScript III First-Strand Synthesis System	Thermo Fisher Scientific	Cat# 18080051
QuikChange II Site-directed Mutagenesis Kit	Agilent Technologies	Cat# 200555
QIAshredder	QIAGEN	Cat# 79654
RNA 6000 Pico Kit	Agilent Technologies	Cat# 5067-1513
QuantiTect SYBR Green PCR kit	QIAGEN	Cat# 204141
QIAamp DNA Mini Kit	QIAGEN	Cat# 51304
QIAquick PCR Purification Kit	QIAGEN	Cat# 28104
High Sensitivity DNA Kit	Agilent Technologies	Cat# 5067-4626
mMESSAGE mMACHINE SP6 Transcription Kit	Thermo Fisher Scientific	Cat# AM1340
Poly(A) Tailing Kit	Thermo Fisher Scientific	Cat# AM1350
Experimental Models: Organisms/Strains
X. laevis	Nasco	https://www.enasco.com/product/LM00715MX/; https://www.enasco.com/product/LM00535MX/
Oligonucleotides
Morpholino: β-actin MO 5'-CAATATCGTCTTCCATTGTGATCTG-3'	Leung et al., 2006; Gene Tools	Xenbase, RRID: SCR_003280: XB-MORPHOLINO-17249112
Morpholino: Control MO 5'-CCTCTTACCTCAGTTACAATTTATA-3'	Gene Tools	N/A
Primer: actb 5' UTR, Venus and the linker 5'-TACTCGGATCCGGCTCAGTGACCCGCCCGCATAGAAAGGAGACAGTCTGTGTGCGTCCAACCCTCAGATCACAATGGTTAGTAAGGGCG-3' 5'-GTATGAATTCAAGCTTTTTGTAAAGTTCATCC-3'	Sigma-Aldrich	N/A
Primer: linker, actb CDS, actb 3' UTR 5'-GCTTGAATTCAAAATGGAAGACGATATTG-3' 5'-CGTAGCGGCCGCGTGAAACAACATAAGT-3'	Sigma-Aldrich	N/A
Primer: morpholino insensitive actb 5'-CAACCCTCAGATCACAATGGAGGATGACATAGCCGCACTGGTCGTTG-3'	Sigma-Aldrich	N/A
Primers for rps13 RT-qPCR: 5'-CTTCAAACTGGCCAAGAAGG-3' 5'-GGCCAGAGCCTTAGACTTGA-3'	Sigma-Aldrich	N/A
Primers for actb RT-qPCR: 5'-TACTCTTTTGTTGGCGCTTG-3' 5'-GGGCAACACTGAGAGGGTAG-3'	Sigma-Aldrich	N/A
Primers for sdha RT-qPCR: 5'-AGACTCAACATGCAGAAGACCA-3' 5'-TCCATTGCAGAATTGATGACAC-3'	Sigma-Aldrich	N/A
Recombinant DNA
pCS2+mGFP	Das et al., 2003	N/A
pCS2+mRFP	Poggi et al., 2005	N/A
Mito-GFP	Michael Coleman (Department of Clinical Neuroscience, University of Cambridge, UK)	N/A
pCS2+MO insensitive β-actin/mGFP dual promoter construct	This paper	N/A
pCS2+Venus-β-actin	This paper	N/A
pCS2+Venus	This paper	N/A
Software and Algorithms
Fiji	Schindelin et al., 2012	Fiji, RRID: SCR_002285
Descriptor-based registration	Preibisch et al., 2010	http://imagej.net/SPIM_Registration
Simple Neurite Tracer	Longair et al., 2011	https://imagej.net/Simple_Neurite_Tracer
Colocalization test	Tony Collins	https://imagej.net/Colocalization_Test
Bleach Correction	Miura et al., 2014	https://imagej.net/Bleach_Correction
Volocity	v.6.3.1	Volocity 3D Image Analysis Software, RRID: SCR_002668
GraphPad PRISM	v.6.01	GraphPad Prism, RRID: SCR_002798
MATLAB	v.R2015b	MATLAB, RRID: SCR_001622
Batch Analysis of Mean and SD. Evolution	This paper; Cambridge repository server	https://doi.org/10.17863/CAM.9542