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. 2005 Apr 4;102(16):5856–5861. doi: 10.1073/pnas.0501634102

Fig. 2.

Fig. 2.

Real-time quantitative RT-PCR analyses of mRNA temporal expression of microglial and astrocytic activation markers in mouse lumbar spinal cord. TaqMan real-time RT-PCR was performed. Total mRNA was isolated from L5 lumbar spinal cord tissue of KO mice (C57BL/10SCNJ) and TLR4 wild-type control mice (C57BL10/ScSnJ). Data are shown for normal, uninjured mice (N; n = 3 per strain) or KO mice (n = 4) and wild-type mice (n = 4) subjected to L5 nerve transection surgery at days 3, 7, and 14 for CD11b (a), CD14 (b), TLR4 (c) and GFAP (d) in the presence of their respective primers and probes. No PCR amplification was observed in the TLR4-deficient mice with TLR4 primers and probe (data not shown). (c) The time-course expression of TLR4 in wild-type mice after injury. The real-time PCR was performed in duplicate for both target gene and GAPDH. The level of gene expression was calculated after normalizing against GAPDH in each sample and is presented as relative mRNA expression units. Values are mean ± SEM. (**, P < 0.01 vs. KO; *, P < 0.05 vs. KO; two-way ANOVA followed by Bonferroni post hoc test.)