Table 2. Fluorescence intensities of 2-OH-E+ and E+ in the presence and absence of DNA at selected wavelengths.
Samples | Ex, nm | Em, nm | Intensity, au |
---|---|---|---|
2-OH-E+ | 510 | 590 | 3.09 |
490 | 590 | 5.55 | |
E+ | 510 | 590 | 2.02 |
490 | 590 | 2.77 | |
2-OH-E+/DNA | 510 | 590 | 61.93 |
490 | 590 | 63.41 | |
494 | 567 | 99.99 | |
E+/DNA | 510 | 590 | 30.95 |
510 | 610 | 24.25 | |
490 | 590 | 25.83 | |
494 | 567 | 18.16 |
2-OH-E+ (10 μM) and E+ (10 μM) were used in the measurement of intensities. Intensities of E+ and 2-OH-E+ were obtained from data shown in ref. 5, and those of E+/DNA and 2-OH-E+/DNA were obtained from this study. Ex, excitation wavelength; Em, emission wavelength; au, arbitrary units.