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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Kidney Int. 2017 Mar 16;92(2):365–376. doi: 10.1016/j.kint.2017.01.020

Figure 8. Effect of high dose (HD) peritoneal dialysis (PD) in ischemic AKI on lung leukocyte infiltration as assessed by flow cytometry.

Figure 8

High dose peritoneal dialysis (PD) was performed one hour after ischemic AKI (AKI) for 4 hours with peritoneal dialysate exchanges occurring every 15 minutes [i.e., 4 exchanges per hour for four hours for a total of 16 exchanges]. Normal mice, and mice 5 hours after ischemic AKI were also studied. Flow cytometry was performed on the entire lung and A) CD45+ cells; B) Neutrophils, C) Interstitial macrophages, D) CD103+ Dendritic cells; E) CD11b+ Dendritic cells, and F) Eosinophils were determined. CD45+ cells, neutrophils and interstitial macrophages were significantly reduced in mice with peritoneal dialysis. G) Representative gating strategy for neutrophil detection. (n= normal (4), AKI (9), AKI + PD (9). *P<0.05 versus AKI; ** P<0.01 vs. Normal; *** P<0.001 versus Normal; NS = not significant versus Normal or AKI; one way ANOVA with Dunnet’s post hoc procedure with AKI as the comparison group. Group numbers are Normal: n=4; AKI: n=9; AKI + PD: n=9.