Figure 2.

Characterization of hGRN‐3 expressed and purified from human embryonic kidney (HEK) cells. (Α) SDS‐PAGE of hGRN‐3 under reduced, non‐reduced, and boiled (lanes 1 and 2, respectively), and reduced, non‐reduced and unboiled (lanes 3 and 4, respectively). The presence of monomer, dimers, and trimers in lanes 2 and 4 are marked with single, double, and triple arrows, respectively. (Β) MALDI‐ToF spectra of hGRN‐3 showing signals corresponding to monomeric hGRN‐3 (single arrow), dimeric hGRN‐3 (double arrows), and trimeric hGRN‐3 (triple arrows) under reduced and non‐reduced conditions. The results are indicative of three consistent repeats.