Figure 6.

Structural stability of GRN‐3, mGRN‐3, and rGRN‐3. (Α) Far‐UV CD spectra without SDS (solid line), and in presence of 1% SDS at 10°C (dotted line), and 90°C (dashed line). (Β) Molar ellipticity difference between 198 nm (random coil) and 208 nm (α‐helix) for GRN‐3 (▪), mGRN‐3 (○), and rGRN‐3 (Δ) plotted as a function of increasing temperature in presence of 1% SDS. (C) SDS‐PAGE under reduced and non‐reduced conditions with heat treatment (lanes 1, 2, 5, 6, 9, and 10, respectively) and reduced and non‐reduced conditions without the heat treatment (lanes 3, 4, 7, 8, 11, and 12, respectively).