FIGURE 5.

M. tuberculosis Ag85B FF15 tetramer⁺ Tregs can be detected in milk from C57BL/6 dams. Dot plot (A) and data plot (B) (n = 6) showing FF15-specific (SP) tetramer⁺CD4⁺Foxp3⁺ Tregs in stomach milk samples from midlactation neonates nursed by an M. tuberculosis–immunized and then challenged dam. Irrelevant tetramer PA15 (NS) was used as a negative control. Gating was first for lymphoid cells, then for CD4⁺, then for Foxp3⁺, and then for tetramer⁺. (B) Data are the percentage of CD4⁺Foxp3⁺ cells also positive for the specific tetramer. All results are presented as mean ± SD. *p < 0.05.