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. 2017 Jul 31;199(5):1875–1885. doi: 10.4049/jimmunol.1700762

FIGURE 3.

FIGURE 3.

Isolation of somatically rearranged L chain from genomic DNA and cDNA. (A) σ L chain amplified from genomic DNA. Left, L chain cluster in GL configuration and after deletional rearrangement. PCR primers, as indicated by arrows, amplified GL band at 878 bp and rearranged at ∼500 bp. Right, The PCR products from Pup1 cells were blotted and hybridized with V region probe. (B) σ L chain amplified from cDNA. Left, Agarose gel with PCR products of two sizes, 549 and ∼488 bp, that are ST1 and rearranged VJ, respectively. Right, The ST1 consists of signal sequence spliced to V gene segment, JL spliced to C region, and the intersegmental sequence spliced at cryptic sites. ST2 is intersegmental cryptic site spliced to the C region 5′ acceptor site. Gray boxes represent signal sequence (L) and V gene segment, darker box is JL, open box is C exon. Black triangle is RSS with 12-bp spacer, open triangle RSS with 23-bp spacer.