Table VI. Analyses of λ mutants over FR and CDR.
| λa | Mutations in |
FR Total |
CDR Total |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FR1 | CDR1 | FR2 | CDR2 | FR3 | CDR3 | FR4 | 268 bp | 81 bp | No. cDNA | |||
| FT | 5 | 33 | 3 | 13 | 37 | 23 | 31 | 76 | 1.2% | 69 | 3.7% | 23 |
| Kplus | 3 | 5 | 3 | 2 | 31 | 8 | 11 | 48 | 1.3% | 15 | 1.3% | 14 |
| Amino Acid Substitutions in V |
|||||||
|---|---|---|---|---|---|---|---|
| FR-R | FR-S | RS Ratio | CDR-R | CDR-S | RS Ratio | CDR-R/FR-R | |
| FT | 38, 1.9% | 23, 1.1% | 1.7 | 45, 7.2% | 8, 1.3% | 5.6 | 7.2/1.9 (3.8) |
| Kplus | 31, 2.5% | 8, 0.6% | 3.9 | 11, 2.9% | 0, n.a. | n.a. | 2.9/2.5 (1.2) |
a
For λ calculations, the entire VJ was used because the NS3-23 gene is GL-joined and in-frame. The CDR consisted of CDR1 (39 bp), CDR2 (21 bp), and the fixed CDR3 (21 bp). The frequencies of FT mutations were calculated as follows: FT, 76/(23 × 268) = 1.2% for FR and 69/(23 × 81) = 3.7% for CDR; Kplus, 48/(14 × 268) = 1.3% for FR and 15/(14 × 81) = 1.3% for CDR.
n.a., not applicable.