Table 1.

Amino acid changes from parental Asibi strain (AY640589) to representative vaccines from each of three substrains 17D-204 (Stamaril®, X15062), 17D-213(YFU17067) and 17DD(YFU1066). Substitutions indicated in grey are conserved between all substrains of 17D and are adapted from the current WHO standard for YFV vaccine production [30].

Position	Gene	Codon	Amino Acid	17-204(X15062)	17D-213(YFU17067)	17DD(YFU17066)
854	M	36	L	F	F	F
1127	E	52	G	R	R	R
1140	E	56	A	V	V
1431	E	153	N		T
1436	E	155	D			S*
1437	E	155	D			S*
1482	E	170	A	V	V	V
1491	E	173	T	I	I	I
1572	E	200	K	T	T	T
1870	E	299	M	I	I	I
1887	E	305	S	F	F	F
1946	E	325	P	S	S
1965	E	331	K	R	R	R
2112	E	380	T	R	R	R
2193	E	407	A	V	V	V
2219	E	416	A	T	T
2220	E	416	A			V
2687	NS1	79	L	F	F	F
3371	NS1	307	I	V	V	V
3860	NS2A	118	M	V	V	V
4007	NS2A	167	T	A	A	A
4013	NS2A	169	L	F	F
4022	NS2A	172	T	A	A	A
4056	NS2A	183	S	F	F	F
4289	NS2B	37	I	L	L	L
4505	NS2B	109	I	L	L	L
5115	NS3	182	Q			R
5153	NS3	195	I	V	V
6023	NS3	485	D	N	N	N
6876	NS4A	146	V	A	A	A
7171	NS4B	95	I	M	M	M
7497	NS4B	204	L		S
7580	NS4B	231	Y	H	H	H
7701	NS5	22	Q	R	R
8808	NS5	391	N			S
10142	NS5	836	E	K	K	K
10338	NS5	901	P	L	L	L
10367	3′UTR	-	T	C	C	C
10418	3′UTR	-	T	C	C	C
10550	3′UTR	-	T	C	C
10722	3′UTR	-	G	A
10800	3′UTR	-	G	A	A	A
10847	3′UTR	-	A	C	C	C

^*Substitution is encoded at two positions at the indicated codon.