Fig. 1.

Evaluation of cell number after ATP treatment on human ESCC cell lines. The cell counting experiments were used as indicative of cell proliferation on KYSE30 (a), KYSE450 (b), and OE21 (c) cell lines. The cells were evaluated after 24, 48, and 72 h under treatment with ATP (1, 3, or 5 mM). The medium DMEM supplemented with 10% of FBS was used a positive control of cell proliferation. The experiments were performed three times in triplicate. Each column represents the mean ± SEM, *p < 0.05, **p < 0.01, and ***p < 0.001, and the results were established in relation to control cells