Table 1.
Primer sequence used to RT-PCR and RT-qPCR analysis
| Gene | Primer sequence RT-qPCR |
|---|---|
| ENTPD1a F | 5′-CTACCCCTTTGACTTCCA-3′ |
| ENTPD1a R | 5′-CTCCCCCAAGGTCCAAAGC-3′ |
| ENTPD2a F | 5′-GGGTGCACGCATCCTCTCG-3′ |
| ENTPD2a R | 5′-CCTCGCTGGCTCTGTCCTC-3′ |
| CD73a F | 5′-GCTGGCGCCTGGGAGCTTAC-3′ |
| CD73a R | 5′-TCCAGCAGCAGCACGTTGGG-3′ |
| P2X7a F | 5′-AGGCAGTGGAAGAGGCCCCC-3′ |
| P2X7a R | 5′-AGTTGTGGCCGGGGAAGTCG-3′ |
| Gene | Primer sequence RT-PCR |
| P2RX1 F | 5′-GCTGGTGCGTAATAAGAAGGTG-3′ |
| P2RX1 R | 5′-ATGAGGCCGCTCGAGGTCTG-3′ |
| P2RX2 F | 5′-AGGTTTGCCAAATACTACAAGATC-3′ |
| P2RX2 R | 5′-GCTGAACTTCCCGGCCTGTC-3′ |
| P2RX3 F | 5′-CTTCACCTATGAGACCACCAAG-3′ |
| P2RX3 R | 5′-CGGTATTTCTCCTCACTCTCTG-3′ |
| P2RX4 F | 5′-GATACCAGCTCAGGAGGAAAAC-3′ |
| P2RX4 R | 5′-GCATCATAAATGCACGACTTGAG-3′ |
| P2RX5 F | 5′-GGCATTCCTGATGGCGCGTG-3′ |
| P2RX5 R | 5′-GGCACCAGGCAAAGATCTCAC-3′ |
| P2RX6 F | 5′-AGCACTGCCGCTATGAACCAC-3′ |
| P2RX6 R | 5′-AGTGAGGCCAGCAGCCAGAG-3′ |
| P2RX7 F | 5′-TGATAAAAGTCTTCGGGATCCGT-3′ |
| P2RX7 R | 5′-TGGACAAATCTGTGAAGTCCATC-3′ |
| GAPDH F | 5′-AACGGATTTGGTCGTATTGGGC-3′ |
| GAPDH R | 5′-CTTGACGGTGCCATGGAATTTG-3′ |
F forward, R reverse
aMelting curve analysis was performed to determine the specificity for each qPCR reaction