Fig. 5.

GUO and/or TMZ effects on apoptosis levels in A172 glioma cells. After confluence, A172 cells were treated with GUO 500 μM, TMZ 500 μM, or with the drug combination. After 48 h, cells were detached, washed and stained with Annexin V and/or propidium iodide, and counted in a flow cytometer. a Illustrative dot plots of flow cytometry. b Quantification of flow cytometer cells sorted. c Mitochondrial membrane potential (ΔΨm) was assessed and quantified. PI propidium iodide. n = 5 of independent experiments for b and c. *p < 0.05 compared to the control group