Fig. 6.

L1 coating alters morphological features at 6 h post implant. A) Probability of cell ramification (1 is ramified, 0 is T-stage) over distance show that the L1 group (black, solid line) has ramified cells closer to the probe than the control group (blue dotted line) in a logistic regression of probability data. Note: as this is a Bernolli Probability Distribution, error bars were not calculated. B) A sample image of microglia labeled as T-stage or ramified. A solid red bar at the left of each image indicates the surface of the probe. Red dotted lines indicate the axis that was used to distinguish the hemispheres facing toward the probe (left side of line) v. away from the probe (right side of line). C) The T-stage index (T-Index) compares the length of the longest probe-facing process to the longest non-probe facing process, with 0 indicating no non-probe facing processes and 1 indicating fully ramified microglia. D) The directionality index (D-Index) compares number of probe facing processes to number of non-probe facing processes, with 0 indicating all probe-facing processes and 1 indicating totally ramified microglia. Data are presented as means ± SEM; all comparisons are made by Welch’s T-tests. * denotes significance, p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)