FIGURE 6.

Set up of Arabidopsis heterotrophic cell suspension culture, starting from the previously established photosynthetic culture. (A,B) Light microscopy observations of suspension-cultured cells, demonstrating the lack of any chlorophyll fluorescence signal (A), as well as the presence of evident Lugol-stained starch granules (white arrowheads) (B). (C) Ultrastructure of amyloplast-containing suspension-cultured cells. a, amyloplasts; cw, cell wall; m, mitochondria; n, nucleus; st, starch; v, vacuole.