Figure 4.

Yeast Ybh3p is not involved in hexadecenal-mediated cell death. (a) Mitochondrial association of Ybh3p is stimulated by acetic acid, but not by hexadecenal. Intracellular localization relative to the MitoTracker-stained mitochondria of a Ybh3-GFP fusion protein expressed in wild type was observed upon acetic acid (100 mM, 1 h at pH 3.0) or hexadecenal (50 μM, 1 h) treatment. (b) Sensitivity to hexadecenal is not altered in ybh3Δ mutants. The growth efficiency upon external addition of hexadecenal was assayed as in Figure 3(a) in wild type and ybh3Δ mutants.