Figure 7.

Immunohistology for CD146 and connexin 43 (Cx 43) of HUVEC and MSC mono- and cocultures in PRP at day 14. Immunohistology was performed on cryosections of mono- and cocultures with 100% MSCs (100 M), 100% HUVECs (100 H), 75% HUVECs–25% MSCs (75 HM), and 50% HUVECs–50% MSCs (50 HM) (seeding density: 2.5 × 10³ cells/μl gel). Negative controls (NC) are shown in the first row. In comparison to the NC, positive signals of CD146 were detectable in the presence of HUVECs, whereas connexin 43 protein levels were only apparent in a mono- or coculture with MSCs. Scale bars = 100 μm.