Fig. 5.

H1152-treated cells show improved capacity to respond to glucose stimulation and maintain glucose homeostasis in vivo. a Blood glucose of mice transplanted with H1152-treated or DMSO-treated cells. The cells were transplanted at day −4. 180 mg/kg STZ was given at day 0. The kidneys were removed at day 134. Error bar is SEM. b GSIS of mice 5 weeks after transplantation with H1152-treated or DMSO-treated cells. The mice were fasted overnight and injected with 3 g/kg D-glucose. Red and blue arrows indicate positive GSIS in mice transplanted with H1152- or DMSO-treated cells, respectively. c IPGTT of mice 5 weeks after transplanted with H1152-treated or DMSO-treated cells. The mice were fasted overnight and injected with 2 g/kg D-glucose. The blood glucose level was measured every 15 min. N = 6 mice for each condition. Fold induction was calculated by dividing the blood glucose level at each time point by the baseline glucose level. Error bar is SEM. d Quantification of immunohistochemistry analysis of grafts of H1152-treated cells or DMSO-treated cells N = 3 independent biological replicates. Error bar is SEM. STZ Streptozotocin. p-values in a were calculated by two-way repeated measures ANOVA with a Bonferroni test. p-values in c were calculated by unpaired two-tailed Student’s t-test. *p < 0.05, **p < 0.01