Fig. 3.

Binding selectivity measurements for different intercalators. a Distribution of blockage positions obtained by applying the FC protocol on H₀ in presence of Echinomycin at 300 nM (upper, number of beads = 93), Actinomycin at 1 μM (middle, number of beads = 174), and Thiocoraline at 100 nM (lower, number of beads = 127). The blockage position for each bead is obtained from 50 to 300 cycles of the FC protocol. Error bars are inversely proportional to the square root of the number of points for each bin. The distribution is fitted to Gaussian functions. b The probability of observing a blockage at each possible dinucleotide step for Echinomycin (upper), Actinomycin (middle), and Thiocoraline (lower), computed from data in a. The results for Echinomycin and Thiocoraline obtained with optical tweezers are also shown for comparison (Supplementary Fig. 6 and ref. ²¹). Error bars are inversely proportional to the square root of the number of points for each bin