Figure 7.

FPND or ROCK inhibitor inhibited atorvastatin-induced ROCK signaling pathways. HUVEC cells were pretreated with either 20 μM FPND or 2.5 μM H1152 for 1 h and then stimulated with 2 μM atorvastatin for 30 min. (a) The expression ratios of phosphorylated MYPT1/total MYPT1, phosphorylated LIMK1/total LIMK1, and phosphorylated confilin/total confilin were detected by western blotting with specific antibodies, as indicated; (b) the data were quantified by the ratio of the band intensity. (c) Inhibition of ROCK significantly prevented atorvastatin-induced rupture of cell–cell junctions on ECs. HUVECs were cultured on the E-Plate in complete medium for 48 h and pretreated with 30 μM FPND or 2.5 μM H1152 for 2 h, followed by washout and incubation with 2 μM atorvastatin for 24 h. ‘Ator’ in figure indicates 2 μM atorvastatin. Data presented in the bar graphs are the mean±S.D. of three independent experiments. *P<0.05, **P<0.01 and ***P<0.005 (versus the atorvastatin-alone group) were considered significantly different.