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Tetramer staining of PBMCs of infected pigs. Example of gating strategy and staining of cells from pig L1 and pig L18 (mismatched pig) at days 0 and 153 post-infection (14 d.p.b.). (a) Singlet and lymphocyte analysis. (b) CD3+CD8high cells are shown. Tetramer 60 (Tet 60) is shown to specifically stain PBMCs from pig L1 at 14 d.p.b. The same tetramer and negative control tetramer (Tet 381) do not stain PBMCs at 0 d.p.i. or PBMCs from the mismatched pig (L18) at any time point.
