Table 1. SwIV peptides in silico predicted to bind with SLA-1*0702 with their respective in vitro binding results.
Strong binding peptides with a Kd less than 1000 nM were used to generate tetramers.
| Peptide sequence | Viral protein of origin (no.) | Position | Virus | Kd (nM) | Tetramer name | |
|---|---|---|---|---|---|---|
| pdmH1N1 | SpH1N1 | |||||
| NADTLCIGY | HA(1) | 16–24 | + | 1 543 | ||
| SLSTASSWSY | HA(2) | 86–95 | + | 35 | 57 | |
| TLYQNNHTY | HA(3) | 207–215 | + | 6 | 66 | |
| YVSVGSSKY | HA(4) | 215–223 | + | 987 | 93 | |
| SVKNGTYDY | HA(5) | 295–303 | + | 20 000 | ||
| GMIDGWYGY | HA(6) | 300–308 | + | 20 000 | ||
| EIGNGCFEFY | HA(7) | 476–485 | + | + | 132 | 63 |
| CPVSGWAIY | NA(1) | 92–100 | + | + | 6 | 55 |
| CPIGEVPSPY | NA(2) | 171–180 | + | + | 37 | 60 |
| GPSNGQASY | NA(3) | 245–253 | + | + | 25 | 59 |
| SVELNAPNY | NA(4) | 266–274 | + | 20 000 | ||
| EMNAPNYHY | NA(5) | 268–278 | + | 231 | 61 | |
| NMDRAVKLY | M1(1) | 92–100 | + | + | 293 | 62 |
| ALASCMGLIY | M1(2) | 123–132 | + | + | 127 | 56 |
| LASCMGLIY | M1(3) | 124–132 | + | + | 9 | 64 |
| VSYAAAAAY | Negative control | 831 | ||||