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. 2017 Aug 18;85(9):e00290-17. doi: 10.1128/IAI.00290-17

FIG 3.

FIG 3

Both native TRP120 and recombinant TRP120 are ubiquitinated and detected by Western immunoblotting. (A) In vitro TRP120 ubiquitination in the presence of Nedd4L using anti-TRP120 (top) and anti-Ub (bottom). The densitometric analysis shows elevated ubiquitinated TRP120 in the presence of Nedd4L compared with control (middle; Student's t test; *, P < 0.05). (B) Uninfected and infected THP-1 cell lysates were subjected to Ub enrichment and Western blot detection of TRP120 ubiquitination before (lanes 1 and 2) and after (lanes 3 and 4) Ub enrichment.