FIG 2.

Expression of DPP4 in periodontopathic bacteria. (A) Aliquots (5 μl) of bacterial cell suspensions (A₆₀₀ = 2.0) of P. gingivalis wild type (Pg), T. forsythia (Tf), P. intermedia (Pi), P. gingivalis KDP136 and NDP200, and S. mutans (Sm) were incubated with 20 μM Gly-Pro-MCA in the absence (−) and presence (+) of 0.25 mM P32/98. Values are shown as the means ± SD (n = 3). (B) Recombinant DPP4 (0.5 μg for Coomassie brilliant blue [CBB] and 0.1 μg for immunoblotting [IB]) were separated by SDS-PAGE and visualized. (C) Whole-cell lysates were separated by SDS-PAGE and detected by IB (30 μg of proteins). Lysates of P. gingivalis (10 μg), T. forsythia (15 μg), P. intermedia (45 μg), and S. mutans (45 μg) were subjected to IB ECL plus detection.