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. 2017 Aug 18;85(9):e00277-17. doi: 10.1128/IAI.00277-17

FIG 5.

FIG 5

MALDI-TOF MS analysis of truncation of GLP-1 and GIP by recombinant DPP4. (A) A 20 μM concentration of GLP-1(7–37) was incubated with 5 ng of PgDPP4 at 37°C. After 30 min, resultant products were analyzed using mass spectrometry. (B) The amounts of the truncated forms GLP-1(9–37) are plotted over time. (C and D) GLP-1 was incubated with 20 ng of TfDPP4 (C) and PiDPP4 (D). (E) A 20 μM concentration of GIP(52–93) (Mr = 4,983.6) was incubated with 5 ng of PgDPP4 at 37°C. (F) The amounts of GIP(54–93) (Mr = 4,749.3) are plotted. (G and H) GIP was incubated with 20 ng of TfDPP4 (G) and PiDPP4 (H). Data are representative of results from at least three independent experiments.