Measurement of culture growth yields and supernatant sialidase activities of F4969, the isogenic nanR null mutant, and the reversed mutant grown for 24 h at 30°C in DMEM without (Con) or with d-glucose (1.6 mM, 16 mM, 80 mM, and 160 mM [G1.6, G16, G80, and G160, respectively]). (A) Growth yields in 24-h cultures grown in the specified media. (B) Sialidase activities in supernatants of the cultures used for panel A. The data shown in both panels are mean values for three independent experiments. The error bars indicate standard deviations. *, P < 0.05 compared to the control by ordinary one-way ANOVA.