FIG 1.

The albumin component of WLM rescues S. aureus (SA) from eradication when it is in coculture with P. aeruginosa (PA). (A) PAO1-SA31 cocultures were grown overnight in Luria-Bertani (LB) broth, full WLM (wound-like medium), or specific components of WLM, including Bolton broth (BB), Bolton broth plus red blood cells (BB+RBC) or plasma, or Bolton broth plus heat-denatured plasma. (B) PAO1-SA31 cocultures were grown overnight in LB broth, full WLM, or LB broth supplemented with the indicated components. Overnight cultures were then serially diluted and plated on Pseudomonas and Staphylococcus isolation agar to determine the number of CFU per milliliter. Plasma was heat denatured by heating whole plasma to 80°C for 15 min. Albumin was depleted from plasma using DEAE Affi-Gel Blue gel columns (Bio-Rad) according to the manufacturer's recommendations. Experiments were repeated at least three times, with at least three technical replicates.