Genetic adjuvantation with dnSHP induces significantly enhanced antigen-specific IFN-γ responses. Mice were vaccinated with each of the adenoviral constructs (caAKT-TC24/TSA1, iMC-TC24/TSA1, and dnSHP-TC24/TSA1), and antigen-specific responses were measured by the IFN-γ secretion of splenocytes stimulated with and without 10 μg/ml Tc24 protein by an ELISpot assay. Compared to caAKT and iMC, genetic adjuvantation with dnSHP induced a significant enhancement of the production of Tc24-specific IFN-γ responses. NS, not significant; *, P ≤ 0.05.