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. 2016 Jan 18;32(1):83–91. doi: 10.1007/s12264-016-0013-1

Fig. 1.

Fig. 1

ROCK2 was expressed in BV2 microglial cells and the ROCK inhibitors were not cytotoxic to BV2 cells. (A) Expression of Iba1 and ROCK2 identified by staining with anti-Iba1 (red) (A1) and anti-ROCK2 (red) (A2). Scale bar, 25 μm. (B) Mean LDH release after 0.5, 1, and 3 h of treatment with Y27632 and fasudil (n = 4). (C) Statistical analysis of ROCK activity in BV2 microglial cells after Y27632 and fasudil treatment. Compared with the control group, ROCK activity was significantly decreased in the Y27632 and fasudil groups (n = 4, *P < 0.01).