Fig. 3.

Y27632 and fasudil changed cell morphology and enhanced uptake activity in primary microglia. (A) Expression of ROCK2 identified by staining with anti-ROCK2 (red) and DAPI (blue). (B) Double staining with Iba1 (red) and DAPI (blue) 1 h after treatment in the control (B1), Y27632 (B2), and fasudil (B3) groups (scale bar, 25 μm. (C) Representative examples of flow cytometric analysis of control, Y27632-, and fasudil-treated primary microglia at 1 h. (D) The data indicated that Y27632 and fasudil stimulated uptake in primary microglia. The percentage of primary microglia that took up FITC-dextran (D1) and the mean fluorescence intensity of FITC-dextran (D2) in primary microglia increased after Y2762 and fasudil treatment. The results are expressed as the mean ± SEM (n = 5, *P < 0.01, Y27632 vs control group, fasudil vs control group).