Effects of Y27632 and fasudil on p-ERK proteins in BV2 microglia. (A) Representative Western blots showing the changes in p-ERK expression in response to Y27632, fasudil, U0126, Y27632 plus U0126, and fasudil plus U0126 treatment at 1 h. (B) Statistical analysis of p-ERK protein expression. Levels of p-ERK were reduced after U0126 treatment at 1 h, although total ERK1/2 expression remained unchanged. The results are expressed as the mean ± SEM (n = 5, *P < 0.01, #
P < 0.05).