Fig. 6.

Involvement of the ERK signaling pathway in the Y27632- and fasudil-induced uptake activity and morphological changes in BV2 microglia. Changes in (A) the percentage of BV2 microglia that took up FITC-dextran and (B) the mean fluorescence intensity of FITC-dextran in BV2 microglia were dependent on p-ERK protein expression. The results are expressed as the mean ± SEM (n = 5, *P < 0.01). (C) Morphological changes of BV2 cells double stained with Iba1 (red) and DAPI (blue) 1 h after treatment in control (C1), Y27632 (C2), fasudil (C3), U0126 (C4), Y27632 plus U0126 (C5), and fasudil plus U0126 (C6) groups (scale bar, 10 μm).