. Author manuscript; available in PMC: 2018 Feb 1.

Published in final edited form as: Innate Immun. 2016 Dec 8;23(2):175–187. doi: 10.1177/1753425916681444

Table 2. Binding of C/EBP-β and PU.1 on the 5′ promoter region of Il1β and Il6 in WT and p65 ^Δmye peritoneal MΦs.

ChIP data reveal that there is a significantly increased binding of C/EBP-β and PU.1 on Il1b and Il6 promoters in p65 ^Δmye peritoneal MΦs. The amount of immunoprecipitated DNA was analyzed by real-time PCR and then normalized over the respective input control.

Transcription factor	Enrichment over negative control (Gapdh intronic region)
	Peritoneal MΦs
	Il1b		Il6
	WT	p65^Δmye	WT	p65^Δmye
C/EBP-β	1.60^(n.s.)	2.64^*	60.02^(n.s.)	266.34^*
PU.1	1.41^(n.s.)	34.73^*	0 ^(n.s.)	336.62^*

Data in the table represent the fold increase of immunoprecipitated DNA for Il1b or Il6 over a negative control DNA fragment (Gapdh intronic region).

p < 0.05 vs. negative control; n=2 for each group;

Il1b: interleukin 1b; Il6: interleukin 6; MΦs: macrophages; n.s.: not significant.

Table 2. Binding of C/EBP-β and PU.1 on the 5′ promoter region of Il1β and Il6 in WT and p65 Δmye peritoneal MΦs.

Table 2. Binding of C/EBP-β and PU.1 on the 5′ promoter region of Il1β and Il6 in WT and p65 ^Δmye peritoneal MΦs.