Skip to main content
. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Nat Cell Biol. 2017 May 1;19(6):698–710. doi: 10.1038/ncb3518

Figure 1. Tissue specific regulation of the IRE1 endonuclease activity in cDCs.

Figure 1

(A) Global gating strategy of cDCs, regardless of tissue origin. Graphs of spleen cDCs are shown. (B) ERAI VenusFP expression in lung (top), lamina propria of small intestine (LP-SI) (middle) and LP-colon (bottom histograms) in T-cells, B-cells and cDCs from ERAI Tg animals. Values depict geometrical mean fluorescence (gMFI) obtained from 1 representative sample. (C) Heat map analysis of ERAI VenusFP fluorescence in cDC1s and cDC2s derived from various organs. Splenic B-cells, T-cells and monocytes were used as immune cell controls for VenusFP levels. cDCs derived from lungs, small intestine and colon are highlighted. Data is representative of 2 independent experiments. (D) Fluorescence measured by flow cytometry of splenic, lung and LP-SI WT cDC1s stained with an antibody raised against IRE1. Bar graphs represent mean gMFI +/− S.E.M (n=4-6-6). Kruskal-Wallis test with Dunn’s multiple comparisons. Data is representative of 3 independent experiments.