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. 2016 Sep 15;32(6):512–522. doi: 10.1007/s12264-016-0061-6

Fig. 3.

Fig. 3

miR-491 modulates DAT expression at the post-transcriptional level through 3′UTR VNTRs. A Effects of miR-491/anti-miR-491 on the activity of the luciferase reporter construct with the SLC6A3 3′UTR in SK-N-SH cells. miR-491 or anti-miRNA (150 nmol/L) was transfected for 48 h. DAT mRNA levels were assessed by quantitative PCR (B), and protein levels were analyzed by Western blot (C) in HepG2 cells transfected with miRNA, anti-miRNA, or ctl-miR (150 nmol/L) for 72 h (representative result from three independent experiments). D The SLC6A3 3′UTR (7 VNTRs) with mutation of the seed region of miR-491. SK-N-SH cells were transfected with different constructs for 5 h, followed by transient transfection with miR-491/anti-miR-491 (150 nmol/L) for 48 h. E Different concentrations of the miR-491 were transfected for 48 h. The effect of ctl-miR on luciferase activity was defined as 1 for each concentration. F Effects of miR-491/anti-miR-491 (150 nmol/L) on the activity of the luciferase reporter constructs with different VNTRs. *P < 0.05; **P < 0.01; ***P < 0.001 vs ctl-miR, NS not significant, WT wild-type.