Fig. 5.

miR-137 and miR-491 regulate DAT-dependent DA uptake into nerve cells. A, B The effects of miR-137 (A) and miR-491 (B) on DA transport in nerve cells. SK-N-SH cells were incubated with 5 μg/mL DA for 5 h after treatment with miR-137/anti-miR-137 (100 nmol/L) (A) or miR-491/anti-miR-491 (150 nmol/L) (B) for 72 h. C After transfection of the DAT-OE plasmid, DA in SK-N-SH cells increased. After transfection with SLC6A3 siRNA, the levels of SLC6A3 mRNA (D) and DA (E) significantly decreased. F SLC6A3 silencing prevented the effects of miR-137 and miR-491 on DA transport. *P < 0.05; **P < 0.01; ***P < 0.001 vs ctl-miR, NS, not significant; DAT-OE, DAT overexpression,