Table 1.

PCR primers and VNTR sequences (h, human).

Name	Sequence	Description
hSLC6A3 forward	5′-ATGAGTAAGAGCAAATGCTCCG-3′	Primers for human SLC6A3 mRNA CDS-3′UTR (coding sequence and 3′UTR); template: cDNA synthesis from SK-N-SH cell total RNA; insertion vector of amplification products: pcDNA3.1/NT-GFP-TOPO
hSLC6A3 reverse	5′-GCAGTTTTTCCATTGTGGATGTC-3′
hSLC6A3 3′UTR forward	5′-AGAGCGGCCGCGCAGTTTTTCCATTGTGGATGTC-3′	Primers for human DAT mRNA 3′UTR; restriction sites: Xho I, Not I; insertion vector of amplification products: psiCHECK2
hSLC6A3 3′UTR reverse	5′-CCGCTCGAGAGGGAGCAGAGACGAAGAC-3′
hSLC6A3 137MUT forward	5′-AGTTTTTGTTTACAAGAATAATTACGATATCTGAGTGAAG-3′	Overlap extension PCR primers for miR-137 seed region mutation
hSLC6A3 137MUT reverse	5′-CTTCACTCAGATATCGTAATTATTCTTGTAAACAAAAACT-3′
hGAPDH forward	5′-TGCACCACCAACTGCTTAGC-3′	Real-time PCR primers for human GAPDH mRNA
hGAPDH reverse	5′-GGCATGGACTGTGGTCATGAG-3′
hSLC6A3 forward	5′-TGTGCTGGAAGCTGGTCA-3′	Real-time PCR primers for human SLC6A3 mRNA
hSLC6A3 reverse	5′-GGGTCTGAAGGTCACAATGC-3′
Human mutation VNTR	5′-AGCGTGTACTACCCCAGGACGCATGCAGGGCCGCGAGA-3′	miR-491 seed region mutation of DAT mRNA VNTR
Human wild-type VNTR	5′-AGCGTGTACTACCCCAGGACGCATGCAGGGCCCCCACA-3′	Basic unit of DAT mRNA VNTR