Extended Data Figure 5. cDNA concentration of amplicon primer pools predicts sequencing outcome.

cDNA concentration of amplicon pools (as measured by Agilent 2200 Tapestation) is highly predictive of amplicon sequencing outcome. On each axis, 1 + primer pool concentration is plotted on a log scale. Each point is a technical replicate of a sample and colours denote observed sequencing outcome of the replicate. If a replicate is predicted to be passing when at least one primer pool concentration is ≥0.8 ng µl⁻¹, then sensitivity is 98.71% and specificity is 90.34%. An accurate predictor of sequencing success early in the sample processing workflow can save resources.