FIG. 2.

Pancreatic gene expression in hAECs under different conditions. A heat map comparing gene expression under the various culture conditions: aA: Nontransduced cells in the presence of Activin A, Nic: Nontransduced cells in the presence of nicotinamide, Pdx: Pdx1-transduced cells, GFP: eGFP-transduced cells, Pdx+aA: Pdx1-transduced cells in the presence of Activin A, GFP+aA: eGFP-transduced cells in the presence of Activin A, Pdx+Nic: Pdx1-transduced cells in the presence of nicotinamide, GFP+Nic: eGFP-transduced cells in the presence of nicotinamide. Gene expression from low to high is represented as a gradation from white to gray. Gene expression is represented as the RQ values (Please refer methodology for RQ calculations). The RQ value of those genes whose expression was undetectable is represented as 0. RQ values corresponding to 1 indicate baseline gene expression in unmanipulated p2 hAECs. All values greater than 1 indicate expression higher than baseline, whereas values <1 indicate lower than baseline expression. N/A indicates that expression of that gene was not studied for that particular sample. RQ, relative quantitation.