Table 1.
Gene (GenBank ID) | F primer sequence (5′–3′) | R primer sequence (5′–3′) | Expected product size |
---|---|---|---|
RFX6 (306518575) | TCTCTTTGACCAGCATGTCG | CTGTGCTGCCTGAAATGGTA | 104 bp spanning region within exon 12 |
CFTR (306514) | CTATGACCCGGATAACAAGGAGG | CAAAAATGGCTGGGTGTAGGA | 107 bp spanning region within exon 4 of all transcript variants |
RFX3 (Harvard PrimerBank ID 19743882c2) | CCAGGTGACTACCGTGGTCT | GCTGCTGATGAGTTGTCCTCC | 88 bp spanning region within exon 1 |
The table lists the genes whose expression was evaluated by the SYBR® Green method. The corresponding forward (F) and reverse (R) primer sequences and the region it spans in the target gene are also mentioned.