Table1.

The PCR programs were used in this study

Factor	Time					Temperature (ºC)
Gene	AdeS		AdeR	OXA-23			AdeS		AdeR	OXA-23
Steps
Initial denaturation	5min		5min	5min			94°C		94ºC	94°C
Denaturation.	1min		1min	25sec			94ºC		94ºC	94ºC
Annealing	1min		1min	40sec			57°C		57°C	56°C
Extension	1 min		1 min	50sec			72ºC		72ºC	72ºC
Final extension	7min		7min	6min			72°C		72°C	72°C
Cycles					30			30			30