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. 2017 Jun 1;117(1):89–101. doi: 10.1038/bjc.2017.156

Figure 3.

Figure 3

Scratch wound healing, proliferation and apoptosis of breast cancer MDA-MB-231 and MCF-7 cells responding to the miRNA mimic treatment. (A and B) Scratch wound healing capacity. Cells were treated with individual miRNA mimics for 48 h. The monolayer of the cells were then scratched with a pipette tip for a line gap. The 48-well plate containing scratched cells were loaded on to an EVOS system and monitored by time lapse. The gap closure was then automatically measured using a home-created macros in ImageJ. (C and D) Proliferation determined with AlamarBlue assay. (E) Apoptosis estimated using Caspase-Glo 3/7 Assay. The data shown are means±s.d. (n=4). *P<0.05 vs mimic control at the same time point. A full colour version of this figure is available at the British Journal of Cancer journal online.