Skip to main content
. 2017 Jun 1;117(1):89–101. doi: 10.1038/bjc.2017.156

Figure 5.

Figure 5

miR-7 mimic exerts an inhibitory effect on post-wound migration, proliferation and In-Vitro angiogenic potential of HMVEC endothelial cells. Cells were pre-treated with miR mimics before the cellular assays. (A) Effect of miR mimics on post-wound migration of HMVECs indicated by the ECIS system. Normalised resistance is proportional to cell migration capacity after electric wound (2600 μA for 20 s). Data are means±s.d. with 6 duplication. (B) Effect of miR mimics on proliferation of HMVECs. AlamarBlue assay was used to estimate proliferation. Data are means±s.d. with 6 duplication. (C) Effect of miR mimics on in-vitro angiogenesis of HMVECs. Tubule formation of the HMVECs on Matrigel was monitored using an EVOS FL imaging system with a × 4 objective. Data are means±s.d. with triple tests with 4 captured images per test. (D and E) Pre-treatment with miR-7 reduces transwell (8-μm pores) invasion and migration capacities of HMVECs with response to the Tumour conditioned medium (TCM) of MDA-MB-231. *P<0.05, **P<0.01, ***P<0.001 vs the mimic control or as indicated with a line. A full colour version of this figure is available at the British Journal of Cancer journal online.