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. 2017 Jun 1;117(1):89–101. doi: 10.1038/bjc.2017.156

Table 1. Primers Used for the Real-Time qRT-PCRa.

Gene Sense primer (5′–3′) Anti-sense primer (5′–3′)
ANGPT2 GTCCACATCAAACTCTAAGGA ACTGAACCTGACCGTACAATGTTAACGTGTAGATGCCA
CXCR4 GCCTCTTTTGCAGATATACAC ACTGAACCTGACCGTACAGGTGGGCAGGAAGATTTTAT
CXCR7 TTCCAAGACTTTTTCTGCCT ACTGAACCTGACCGTACATGCGCAAGCTATAAACAAAG
EGFR AGAGTCTCAAAGCCATGTT ACTGAACCTGACCGTACACCATCCTAAGCATGACTCC
ERK ACACGCAGTTGCAGTACA ACTGAACCTGACCGTACAGGGGCTGATCTTCTTGAT
GAPDH CTGAGTACGTCGTGGAGTC ACTGAACCTGACCGTACACAGAGATGATGACCCTTTTG
GROα TCTTCCGCTCCTCTCAC ACTGAACCTGACCGTACACGGACGCTCCTGCTG
IGF1R GTGTTCTTCTATGTCCAGGC ACTGAACCTGACCGTACAAGACGTACAGCATAATCACC
mTOR GCTGCAGAAGAAGGTCACT ACTGAACCTGACCGTACAAAGGAGATGGAACGGAAG
STAT3 CATGGAAGAATCCAACAACG ACTGAACCTGACCGTACAAATCAGGGAAGCATCACAAT
THBS1 ACCAACCGCATTCCAGAGTC ACTGAACCTGACCGTACATCAGGTTGGCATCCTCGAT
VEGFA GAGCCGGAGAGGGAG ACTGAACCTGACCGTACACTGGGACCACTTGGCAT
WAVE3 TGACACCATACAGAGATGAC ACTGAACCTGACCGTACACTGTTAGGGTTCAGCTTGTG
a

The z-sequence (which binds to the 3′-end of the Amplifluor probes) are underlined.