Table 1. Primers Used for the Real-Time qRT-PCRa.
Gene | Sense primer (5′–3′) | Anti-sense primer (5′–3′) |
---|---|---|
ANGPT2 | GTCCACATCAAACTCTAAGGA | ACTGAACCTGACCGTACAATGTTAACGTGTAGATGCCA |
CXCR4 | GCCTCTTTTGCAGATATACAC | ACTGAACCTGACCGTACAGGTGGGCAGGAAGATTTTAT |
CXCR7 | TTCCAAGACTTTTTCTGCCT | ACTGAACCTGACCGTACATGCGCAAGCTATAAACAAAG |
EGFR | AGAGTCTCAAAGCCATGTT | ACTGAACCTGACCGTACACCATCCTAAGCATGACTCC |
ERK | ACACGCAGTTGCAGTACA | ACTGAACCTGACCGTACAGGGGCTGATCTTCTTGAT |
GAPDH | CTGAGTACGTCGTGGAGTC | ACTGAACCTGACCGTACACAGAGATGATGACCCTTTTG |
GROα | TCTTCCGCTCCTCTCAC | ACTGAACCTGACCGTACACGGACGCTCCTGCTG |
IGF1R | GTGTTCTTCTATGTCCAGGC | ACTGAACCTGACCGTACAAGACGTACAGCATAATCACC |
mTOR | GCTGCAGAAGAAGGTCACT | ACTGAACCTGACCGTACAAAGGAGATGGAACGGAAG |
STAT3 | CATGGAAGAATCCAACAACG | ACTGAACCTGACCGTACAAATCAGGGAAGCATCACAAT |
THBS1 | ACCAACCGCATTCCAGAGTC | ACTGAACCTGACCGTACATCAGGTTGGCATCCTCGAT |
VEGFA | GAGCCGGAGAGGGAG | ACTGAACCTGACCGTACACTGGGACCACTTGGCAT |
WAVE3 | TGACACCATACAGAGATGAC | ACTGAACCTGACCGTACACTGTTAGGGTTCAGCTTGTG |
The z-sequence (which binds to the 3′-end of the Amplifluor probes) are underlined.