Table 1. Methanomassiliicoccales OTUs predominantly associated with the human gut microbiome across multiple cohorts.
| Reference | Method | Target gene | Cohort origin | Number of subjects | Age range | Number of OTUs | Occurrence of OTUs found across >4 studies |
|---|---|---|---|---|---|---|---|
| Mihajlovski et al. (2008) | PCR cloning | 16S/mcrA | France | 6 | 25–69 | 1 | Minta/Mlum |
| Scanlan et al. (2008) | PCR cloning | mcrA | Ireland | 207 | 19–74 | 1 | Mint/Mlum |
| Mihajlovski et al. (2010) | PCR cloning | 16S/mcrA | France | 60 | 0–90 | 5 | Mint/Mlum, Malvb, Mx-02, Mx-03, Mx-04 |
| Dridi et al. (2012a) | qPCR | 16S | France | 500 | 0–100 | — | Mint/Mlum?c |
| Hoffman et al. (2013) | NGS on amplicons | 16S | USA | 96 | 18–40 | 3 | Mint/Mlum, Mx-06 |
| Nielsen et al. (2014) | Metagenomic | — | Denmark/Spain | 396 | 18–70 | 1 | Malv |
| Goodrich et al. (2014) | NGS on amplicons | 16S | UK | 977 | 23–86 | 5 | Mint/Mlum, Mx-02, Mx-03, Mx-06 |
| Clemente et al. (2015) | NGS on amplicons | 16S | Amerindian | 12 | 7–48 | 3 | Mx-02, Mx-06 |
| Vanderhaeghen et al. (2015) | PCR cloning | 16S | Switzerland | 44 | 8–78 | 1 | Mx-02 |
| Pozuelo et al. (2015) | NGS on amplicons | 16S | Spain | 179 | 18–86 | 5 | Mint/Mlum, Malv, Mx-06 |
| This study | Metagenomic | — | Ireland | 190 | 64–102 | 5 | Mint/Mlum, Malv, Mx-02, Mx-03, Mx-06 |
Abbreviations: NGS, next-generation sequencing; OTU, Operational Taxonomic Unit; qPCR, quantitative PCR. For the ‘Ca. Mmc. intestinalis’/Mmc. luminyensis OTU, ‘Mint’ was underlined when the sequences were identical or closer to ‘Ca. Mmc. intestinalis’ than Mmc. luminyensis.
a
Named Mx-01.
b
Mx-05 in this early study.
c
Presence of Mmc. luminyensis was determined with primers and probe having only one mismatch with ‘Ca. Mmc. intestinalis’. Mint, ‘Ca. Mmc. intestinalis’ Mlum, Mmc. luminyensis; Malv, ‘Ca. Mmp. alvus’. Presence of ‘Ca. Mmc. intestinalis’ in Scanlan et al. (2008) and Mihajlovski et al. (2010) was inferred by identification of mcrA sequences having >99% nucleotide identity with the mcrA sequence of this species.