Skip to main content
. 2017 Jun 6;11(9):2124–2140. doi: 10.1038/ismej.2017.77

Figure 1.

Figure 1

Methane-dependent growth of Crenothrix in Lake Rotsee and Lake Zug. (a) Crenothrix in the Lake Rotsee oxic incubation visualized by CARD-FISH (green; counterstained by DAPI in blue) with a specific probe Creno445 (Stoecker et al., 2006). A small coccoid cell targeted by the probe (marked by the asterisk) might represent a gonidial cell, which Crenothrix is reportedly capable of producing (Völker et al., 1977). (b) The corresponding 13C/12C nanoSIMS image shows homogeneous 13C enrichment throughout the cell filament. The small coccoid cell is also significantly enriched, albeit less. (c) The corresponding 32S/12C nanoSIMS image showing distribution of organic material on the filter. (d) Putative Crenothrix filaments in the Lake Zug oxic incubation visualized by DAPI (blue) and CARD-FISH (green) with probe Mgamma669. (e) Corresponding 13C/12C and (f) 32S/12C nanoSIMS images. Note the fragmented nature of the Crenothrix filaments and the attached small (unidentified) bacteria. (g) Putative Crenothrix filaments in the Lake Zug anoxic incubation visualized by DAPI (blue) and CARD-FISH (green) with probe Mgamma669. (h) Corresponding 13C/12C and (i) 32S/12C nanoSIMS images.