Figure 7. Galectin-1 effects on arginase-1 and iNOS are ERK-independent, while IL-6 inhibition is ERK-dependent.

Bone marrow-derived macrophages were cultured on the indicated scaffolds as described in Figure 3, and Western blotting was used to measure ERK phosphorylation (A) and levels of arginase-1 and iNOS protein (B–D). (B) shows a representative blot, while (C–D) are normalized values. In (E) IL-6 secretion was measured by ELISA. Data shown are means ± SEM of 3 independent cultures from one of two experiments. * indicates a difference when comparing 60 mg/ml within each group (p < 0.05). # represents a difference compared to 60 mg/ml+Gal1 within each group (p < 0.05). $ represents a difference between Vehicle and ERK inhibitor (p < 0.05).