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. Author manuscript; available in PMC: 2018 Jul 6.
Published in final edited form as: Nature. 2017 Jun 28;547(7661):55–60. doi: 10.1038/nature22992

Extended Data Figure 6. Increased binding of E2F/DP1 to the mutant FOXA1 promoter.

Extended Data Figure 6

a, Immunoblot for haemagglutinin (HA)-tagged E2F3 and DP1 shows binding of both proteins in HEK293T cells transfected with either WT or mutant FOXA1 promoter luciferase construct. Immunoblot against tubulin serves as loading control. b, EMSA for HEK239T cells transfected with E2F3/DP1 expression constructs. EMSA was then performed for FOXA1 WT (lanes 1–3) and mutant (lanes 4–6) promoter probes. Ectopic expression of E2F3/DP1 increases nuclear protein binding signal to the mutant promoter compared with WT (compare lane 6 with lane 3), suggesting that increase in binding observed in mutant over WT is at least in part because of increased recruitment of the E2F/DP1 complex.