a, Sequencing read alignment for tumour BDD-162 shows location of TBC1D12 hotspot mutations on mutually exclusive alleles. b, Location of hotspot mutations near the LEPROTL1 transcription start on mutually exclusive sequencing reads in patient BDD-MEX-BR-116. Reference bases are indicated in grey, mismatched bases in their respective colours (A, green; C, blue; G, orange; T, red). Hotspot mutation sites are outlined with black boxes. Images generated with the Integrative Genomics Viewer70.